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human il 2  (Miltenyi Biotec)


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    Miltenyi Biotec human il 2
    Human Il 2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il/Human+IL-2+IS%2C+premium+grade/pm42906542-1034-37-39
    Average 96 stars, based on 286 article reviews
    human il 2 - by Bioz Stars, 2026-11
    96/100 stars

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    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 16,16-Dimethyl Prostaglandin E2 Cayman Chemical Cat#14750 7-AAD Viability Staining solution Biolegend Cat#420403 ABE8.20-m Fiumara et al. N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 Integrated DNA Technologies Cat#1072544 Cas9 enhancing Integrated DNA Technologies Cat#1075916 CellROXTM Deep Red Reagent Thermo Fisher Cat#C10422 CometAssay LMAgarose R&D Systems Cat#4250-050-02 Corning® Collagen I Sigma-Aldrich Cat#CLS354236-1EA CometAssay Lysis Solution R&D Systems Cat#4250-050-01 DAPI Sigma-Aldrich Cat#D9542 Erythropoietin (EPO, Eprex) Janssen-Cilag N/A gRNA AAVS1 Schiroli et al. N/A gRNA B2M exon 1 Fiumara et al. N/A GSE56 and Ad5-E4orf6/7 Ferrari et al. N/A Hoechst 3342 Fluorescent Stain Thermo Fisher Cat#62249 Human GM-CSF Miltenyi Biotec Cat#130-093-862 Human IL-11 Miltenyi Biotec Cat130-103-439 Human IL-2 Miltenyi Biotec Cat#130097743 Human IL-3 recombinant protein Peprotech Cat#200-03 Human IL-7 Miltenyi Biotec Cat#130-095-363 Human Low-Density Lipoproteins (LDL) Stem Cell Technologies Cat#02698 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 LiberaseTM Sigma-Aldrich Cat#5401119001 nicking gRNA 5 B2M exon 1 Fiumara et al. N/A Pacific Blue Annexin V Biolegend Cat#640918 Paraformaldehyde solution 4% in PBS Santa Cruz Biotechnology Cat#SC-281692 PE3max Fiumara et al. N/A pegRNA 5 B2M exon 1 Fiumara et al. N/A Poly-L-lysine solution Sigma-Aldrich Cat#P8920 Recombinant human Flt3-Ligand Peprotech Cat#300-19 Recombinant human IL6 Peprotech Cat#200-06 Recombinant human stem cell factor (SCF) Peprotech Cat#300-07 Recombinant human thrombopoietin (TPO) Peprotech Cat#300-18 SpCas9 Nuclease Aldevron Cat#9212 StemRegenin 1 (SR1) Biovision Cat#1967 UM171 STEMCell Technologies Cat#72914 Critical commercial assays CellTraceTM Violet Cell Proliferation Kit Thermo Scientific Cat# C34557 Click-iT EdU Pacific Blue Flow cyometry assay kit Thermo Fisher Cat#C10418 ddPCR Supermix for Probes (No dUTP) BioRad Cat #1863024 ddPCRTM Copy Number Assay: TTC5, Human, Homo sapiens BioRad Cat# 12017424 GoTaq Flexi DNA Polymerase Promega Corporation Cat#M8295 P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 QIAamp DNA Micro Kit QIAGEN Cat#56304 (Continued on next page) ll OPEN ACCESS Clinical and Translational Report e2 Cell Stem Cell 33, 1–16.e1–e9, February 5, 2026 .. NOD-SCID-IL2rg− /− (NSG) female mice were purchased from Charles River Laboratories and maintained in specific pathogen-free conditions.

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: .. The cancer cells were incubated for 4 h with or without recombinant human IL-2 IS of research grade (2 μg/mL) (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, the cells were washed with the binding buffer and incubated with Annexin V-FITC (5 μL/tube, 1:20) (Miltenyi Biotec, Bergisch Gladbach, Germany) for 15 min in the dark at RT.

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: .. RCC cells were incubated overnight with or without recombinant human IL-2 IS of research grade (1.16 μg/mL) (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Staining:

    Article Title: Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 16,16-Dimethyl Prostaglandin E2 Cayman Chemical Cat#14750 7-AAD Viability Staining solution Biolegend Cat#420403 ABE8.20-m Fiumara et al. N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 Integrated DNA Technologies Cat#1072544 Cas9 enhancing Integrated DNA Technologies Cat#1075916 CellROXTM Deep Red Reagent Thermo Fisher Cat#C10422 CometAssay LMAgarose R&D Systems Cat#4250-050-02 Corning® Collagen I Sigma-Aldrich Cat#CLS354236-1EA CometAssay Lysis Solution R&D Systems Cat#4250-050-01 DAPI Sigma-Aldrich Cat#D9542 Erythropoietin (EPO, Eprex) Janssen-Cilag N/A gRNA AAVS1 Schiroli et al. N/A gRNA B2M exon 1 Fiumara et al. N/A GSE56 and Ad5-E4orf6/7 Ferrari et al. N/A Hoechst 3342 Fluorescent Stain Thermo Fisher Cat#62249 Human GM-CSF Miltenyi Biotec Cat#130-093-862 Human IL-11 Miltenyi Biotec Cat130-103-439 Human IL-2 Miltenyi Biotec Cat#130097743 Human IL-3 recombinant protein Peprotech Cat#200-03 Human IL-7 Miltenyi Biotec Cat#130-095-363 Human Low-Density Lipoproteins (LDL) Stem Cell Technologies Cat#02698 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 LiberaseTM Sigma-Aldrich Cat#5401119001 nicking gRNA 5 B2M exon 1 Fiumara et al. N/A Pacific Blue Annexin V Biolegend Cat#640918 Paraformaldehyde solution 4% in PBS Santa Cruz Biotechnology Cat#SC-281692 PE3max Fiumara et al. N/A pegRNA 5 B2M exon 1 Fiumara et al. N/A Poly-L-lysine solution Sigma-Aldrich Cat#P8920 Recombinant human Flt3-Ligand Peprotech Cat#300-19 Recombinant human IL6 Peprotech Cat#200-06 Recombinant human stem cell factor (SCF) Peprotech Cat#300-07 Recombinant human thrombopoietin (TPO) Peprotech Cat#300-18 SpCas9 Nuclease Aldevron Cat#9212 StemRegenin 1 (SR1) Biovision Cat#1967 UM171 STEMCell Technologies Cat#72914 Critical commercial assays CellTraceTM Violet Cell Proliferation Kit Thermo Scientific Cat# C34557 Click-iT EdU Pacific Blue Flow cyometry assay kit Thermo Fisher Cat#C10418 ddPCR Supermix for Probes (No dUTP) BioRad Cat #1863024 ddPCRTM Copy Number Assay: TTC5, Human, Homo sapiens BioRad Cat# 12017424 GoTaq Flexi DNA Polymerase Promega Corporation Cat#M8295 P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 QIAamp DNA Micro Kit QIAGEN Cat#56304 (Continued on next page) ll OPEN ACCESS Clinical and Translational Report e2 Cell Stem Cell 33, 1–16.e1–e9, February 5, 2026 .. NOD-SCID-IL2rg− /− (NSG) female mice were purchased from Charles River Laboratories and maintained in specific pathogen-free conditions.

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: The absorbance was measured at 570 nm using Multiskan SkyHigh (ThermoFisher Scientific, Waltham, MA, USA) after 5 min, 1 h, and overnight. .. Live-or-Dye TM Fixable Viability Staining Kit (Biotium, Fremont, CA, USA) was also used to investigate the viability of the four RCC cell lines cultured with human IL-2 IS of research grade (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    CRISPR:

    Article Title: Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 16,16-Dimethyl Prostaglandin E2 Cayman Chemical Cat#14750 7-AAD Viability Staining solution Biolegend Cat#420403 ABE8.20-m Fiumara et al. N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 Integrated DNA Technologies Cat#1072544 Cas9 enhancing Integrated DNA Technologies Cat#1075916 CellROXTM Deep Red Reagent Thermo Fisher Cat#C10422 CometAssay LMAgarose R&D Systems Cat#4250-050-02 Corning® Collagen I Sigma-Aldrich Cat#CLS354236-1EA CometAssay Lysis Solution R&D Systems Cat#4250-050-01 DAPI Sigma-Aldrich Cat#D9542 Erythropoietin (EPO, Eprex) Janssen-Cilag N/A gRNA AAVS1 Schiroli et al. N/A gRNA B2M exon 1 Fiumara et al. N/A GSE56 and Ad5-E4orf6/7 Ferrari et al. N/A Hoechst 3342 Fluorescent Stain Thermo Fisher Cat#62249 Human GM-CSF Miltenyi Biotec Cat#130-093-862 Human IL-11 Miltenyi Biotec Cat130-103-439 Human IL-2 Miltenyi Biotec Cat#130097743 Human IL-3 recombinant protein Peprotech Cat#200-03 Human IL-7 Miltenyi Biotec Cat#130-095-363 Human Low-Density Lipoproteins (LDL) Stem Cell Technologies Cat#02698 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 LiberaseTM Sigma-Aldrich Cat#5401119001 nicking gRNA 5 B2M exon 1 Fiumara et al. N/A Pacific Blue Annexin V Biolegend Cat#640918 Paraformaldehyde solution 4% in PBS Santa Cruz Biotechnology Cat#SC-281692 PE3max Fiumara et al. N/A pegRNA 5 B2M exon 1 Fiumara et al. N/A Poly-L-lysine solution Sigma-Aldrich Cat#P8920 Recombinant human Flt3-Ligand Peprotech Cat#300-19 Recombinant human IL6 Peprotech Cat#200-06 Recombinant human stem cell factor (SCF) Peprotech Cat#300-07 Recombinant human thrombopoietin (TPO) Peprotech Cat#300-18 SpCas9 Nuclease Aldevron Cat#9212 StemRegenin 1 (SR1) Biovision Cat#1967 UM171 STEMCell Technologies Cat#72914 Critical commercial assays CellTraceTM Violet Cell Proliferation Kit Thermo Scientific Cat# C34557 Click-iT EdU Pacific Blue Flow cyometry assay kit Thermo Fisher Cat#C10418 ddPCR Supermix for Probes (No dUTP) BioRad Cat #1863024 ddPCRTM Copy Number Assay: TTC5, Human, Homo sapiens BioRad Cat# 12017424 GoTaq Flexi DNA Polymerase Promega Corporation Cat#M8295 P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 QIAamp DNA Micro Kit QIAGEN Cat#56304 (Continued on next page) ll OPEN ACCESS Clinical and Translational Report e2 Cell Stem Cell 33, 1–16.e1–e9, February 5, 2026 .. NOD-SCID-IL2rg− /− (NSG) female mice were purchased from Charles River Laboratories and maintained in specific pathogen-free conditions.

    Negative Control:

    Article Title: Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 16,16-Dimethyl Prostaglandin E2 Cayman Chemical Cat#14750 7-AAD Viability Staining solution Biolegend Cat#420403 ABE8.20-m Fiumara et al. N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 Integrated DNA Technologies Cat#1072544 Cas9 enhancing Integrated DNA Technologies Cat#1075916 CellROXTM Deep Red Reagent Thermo Fisher Cat#C10422 CometAssay LMAgarose R&D Systems Cat#4250-050-02 Corning® Collagen I Sigma-Aldrich Cat#CLS354236-1EA CometAssay Lysis Solution R&D Systems Cat#4250-050-01 DAPI Sigma-Aldrich Cat#D9542 Erythropoietin (EPO, Eprex) Janssen-Cilag N/A gRNA AAVS1 Schiroli et al. N/A gRNA B2M exon 1 Fiumara et al. N/A GSE56 and Ad5-E4orf6/7 Ferrari et al. N/A Hoechst 3342 Fluorescent Stain Thermo Fisher Cat#62249 Human GM-CSF Miltenyi Biotec Cat#130-093-862 Human IL-11 Miltenyi Biotec Cat130-103-439 Human IL-2 Miltenyi Biotec Cat#130097743 Human IL-3 recombinant protein Peprotech Cat#200-03 Human IL-7 Miltenyi Biotec Cat#130-095-363 Human Low-Density Lipoproteins (LDL) Stem Cell Technologies Cat#02698 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 LiberaseTM Sigma-Aldrich Cat#5401119001 nicking gRNA 5 B2M exon 1 Fiumara et al. N/A Pacific Blue Annexin V Biolegend Cat#640918 Paraformaldehyde solution 4% in PBS Santa Cruz Biotechnology Cat#SC-281692 PE3max Fiumara et al. N/A pegRNA 5 B2M exon 1 Fiumara et al. N/A Poly-L-lysine solution Sigma-Aldrich Cat#P8920 Recombinant human Flt3-Ligand Peprotech Cat#300-19 Recombinant human IL6 Peprotech Cat#200-06 Recombinant human stem cell factor (SCF) Peprotech Cat#300-07 Recombinant human thrombopoietin (TPO) Peprotech Cat#300-18 SpCas9 Nuclease Aldevron Cat#9212 StemRegenin 1 (SR1) Biovision Cat#1967 UM171 STEMCell Technologies Cat#72914 Critical commercial assays CellTraceTM Violet Cell Proliferation Kit Thermo Scientific Cat# C34557 Click-iT EdU Pacific Blue Flow cyometry assay kit Thermo Fisher Cat#C10418 ddPCR Supermix for Probes (No dUTP) BioRad Cat #1863024 ddPCRTM Copy Number Assay: TTC5, Human, Homo sapiens BioRad Cat# 12017424 GoTaq Flexi DNA Polymerase Promega Corporation Cat#M8295 P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 QIAamp DNA Micro Kit QIAGEN Cat#56304 (Continued on next page) ll OPEN ACCESS Clinical and Translational Report e2 Cell Stem Cell 33, 1–16.e1–e9, February 5, 2026 .. NOD-SCID-IL2rg− /− (NSG) female mice were purchased from Charles River Laboratories and maintained in specific pathogen-free conditions.

    Lysis:

    Article Title: Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 16,16-Dimethyl Prostaglandin E2 Cayman Chemical Cat#14750 7-AAD Viability Staining solution Biolegend Cat#420403 ABE8.20-m Fiumara et al. N/A Alt-R CRISPR-Cas9 Negative Control crRNA #1 Integrated DNA Technologies Cat#1072544 Cas9 enhancing Integrated DNA Technologies Cat#1075916 CellROXTM Deep Red Reagent Thermo Fisher Cat#C10422 CometAssay LMAgarose R&D Systems Cat#4250-050-02 Corning® Collagen I Sigma-Aldrich Cat#CLS354236-1EA CometAssay Lysis Solution R&D Systems Cat#4250-050-01 DAPI Sigma-Aldrich Cat#D9542 Erythropoietin (EPO, Eprex) Janssen-Cilag N/A gRNA AAVS1 Schiroli et al. N/A gRNA B2M exon 1 Fiumara et al. N/A GSE56 and Ad5-E4orf6/7 Ferrari et al. N/A Hoechst 3342 Fluorescent Stain Thermo Fisher Cat#62249 Human GM-CSF Miltenyi Biotec Cat#130-093-862 Human IL-11 Miltenyi Biotec Cat130-103-439 Human IL-2 Miltenyi Biotec Cat#130097743 Human IL-3 recombinant protein Peprotech Cat#200-03 Human IL-7 Miltenyi Biotec Cat#130-095-363 Human Low-Density Lipoproteins (LDL) Stem Cell Technologies Cat#02698 Intracellular Staining Permeabilization Wash Buffer Biolegend Cat#421002 LiberaseTM Sigma-Aldrich Cat#5401119001 nicking gRNA 5 B2M exon 1 Fiumara et al. N/A Pacific Blue Annexin V Biolegend Cat#640918 Paraformaldehyde solution 4% in PBS Santa Cruz Biotechnology Cat#SC-281692 PE3max Fiumara et al. N/A pegRNA 5 B2M exon 1 Fiumara et al. N/A Poly-L-lysine solution Sigma-Aldrich Cat#P8920 Recombinant human Flt3-Ligand Peprotech Cat#300-19 Recombinant human IL6 Peprotech Cat#200-06 Recombinant human stem cell factor (SCF) Peprotech Cat#300-07 Recombinant human thrombopoietin (TPO) Peprotech Cat#300-18 SpCas9 Nuclease Aldevron Cat#9212 StemRegenin 1 (SR1) Biovision Cat#1967 UM171 STEMCell Technologies Cat#72914 Critical commercial assays CellTraceTM Violet Cell Proliferation Kit Thermo Scientific Cat# C34557 Click-iT EdU Pacific Blue Flow cyometry assay kit Thermo Fisher Cat#C10418 ddPCR Supermix for Probes (No dUTP) BioRad Cat #1863024 ddPCRTM Copy Number Assay: TTC5, Human, Homo sapiens BioRad Cat# 12017424 GoTaq Flexi DNA Polymerase Promega Corporation Cat#M8295 P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 QIAamp DNA Micro Kit QIAGEN Cat#56304 (Continued on next page) ll OPEN ACCESS Clinical and Translational Report e2 Cell Stem Cell 33, 1–16.e1–e9, February 5, 2026 .. NOD-SCID-IL2rg− /− (NSG) female mice were purchased from Charles River Laboratories and maintained in specific pathogen-free conditions.

    Cell Culture:

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: The absorbance was measured at 570 nm using Multiskan SkyHigh (ThermoFisher Scientific, Waltham, MA, USA) after 5 min, 1 h, and overnight. .. Live-or-Dye TM Fixable Viability Staining Kit (Biotium, Fremont, CA, USA) was also used to investigate the viability of the four RCC cell lines cultured with human IL-2 IS of research grade (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: The amount of synthesized DNA incorporating the thymidine analog BrdU was detected using the microplate reader Multiskan SkyHigh (ThermoFisher Scientific, Waltham, MA, USA) at 370 nm. .. CellTiter 96 ® Non-Radioactive Cell Proliferation Assay (MTT) (Promega, Madison, WI, USA) was used to determine cell viability of A-498, ACHN, Caki-1, and Caki-2 cell lines cultured with human IL-2 IS of research grade (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Incubation:

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: .. The cancer cells were incubated for 4 h with or without recombinant human IL-2 IS of research grade (2 μg/mL) (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, the cells were washed with the binding buffer and incubated with Annexin V-FITC (5 μL/tube, 1:20) (Miltenyi Biotec, Bergisch Gladbach, Germany) for 15 min in the dark at RT.

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: .. RCC cells were incubated overnight with or without recombinant human IL-2 IS of research grade (1.16 μg/mL) (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Proliferation Assay:

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: The amount of synthesized DNA incorporating the thymidine analog BrdU was detected using the microplate reader Multiskan SkyHigh (ThermoFisher Scientific, Waltham, MA, USA) at 370 nm. .. CellTiter 96 ® Non-Radioactive Cell Proliferation Assay (MTT) (Promega, Madison, WI, USA) was used to determine cell viability of A-498, ACHN, Caki-1, and Caki-2 cell lines cultured with human IL-2 IS of research grade (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    MTT Assay:

    Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death
    Article Snippet: The amount of synthesized DNA incorporating the thymidine analog BrdU was detected using the microplate reader Multiskan SkyHigh (ThermoFisher Scientific, Waltham, MA, USA) at 370 nm. .. CellTiter 96 ® Non-Radioactive Cell Proliferation Assay (MTT) (Promega, Madison, WI, USA) was used to determine cell viability of A-498, ACHN, Caki-1, and Caki-2 cell lines cultured with human IL-2 IS of research grade (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Magnetic Cell Separation:

    Article Title: Multiplexed high-content imaging uncovers morphological diversity of lymphocyte activation and dysfunction
    Article Snippet: CD8 + T cells were isolated from healthy donors using the REAlease CD8 MicroBead Kit (Miltenyi Biotec, #130-117-036) according to the manufacturer’s instructions. .. Cells were counted and seeded at a density of 1×10 6 per mL in complete media: Tex MACS media supplemented with 1% human serum and 100 U/mL human IL-2 IS (Miltenyi Biotec, 130-097-748). .. Initial activation is with 1:100 T Cell TransAct (Miltenyi Biotec, #130-111-160) for 72h, then for exhausted cells they are re-stimulated every 48h.



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    Miltenyi Biotec human il 7
    Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin <t>(IL)-4/IL-13</t> or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.
    Human Il 7, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human il 15  (Miltenyi Biotec)
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    Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin <t>(IL)-4/IL-13</t> or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.
    Human Il 15, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    il 1β  (Elabscience Biotechnology)
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    Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin <t>(IL)-4/IL-13</t> or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.
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    Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin <t>(IL)-4/IL-13</t> or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.
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    human il  (ABclonal Biotechnology)
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    Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin <t>(IL)-4/IL-13</t> or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.
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    Image Search Results


    The Impact of IL‐35 on Serum Lipid Profiles. (A) Serum lipid profiles including TG, TC, LDL‐C, and HDL‐C, n = 7. Data are mean ± SD. ns, not significant; *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: The Impact of IL‐35 on Serum Lipid Profiles. (A) Serum lipid profiles including TG, TC, LDL‐C, and HDL‐C, n = 7. Data are mean ± SD. ns, not significant; *** p < 0.001, **** p < 0.0001.

    Article Snippet: HUVECs were assigned to the following groups: control, PA (100 μM), PA + IL‐35 (50 ng/mL), PA + IL‐35 (100 ng/mL), PA + IL‐35 + sorafenib (5 μmol/L, Raf inhibitor, MCE, USA), PA + IL‐35 + SCH772984 (0.5 μmol/L, ERK inhibitor, MCE, USA), PA + IL‐35 + si‐NC (50 nM), and PA + IL‐35 + si‐STAT3 (50 nM), with a total treatment duration of 24 h. PA, IL‐35 and inhibitors were added simultaneously for the control, PA, PA + IL‐35, PA + IL‐35+sorafenib and PA + IL‐35 + SCH772984 groups.

    Techniques:

    IL‐35 Subunit EBI3 is Downregulated in both Obese Mouse Aortas and PA‐Treated HUVECs, and Restored by Exogenous IL‐35. (A, B) Immunofluorescence of EBI3 (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (C, D) Immunofluorescence of IL‐12A (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (E) Serum IL‐35 concentrations, n = 8. (F, H) Representative Western blot bands of EBI3 and IL‐12A in aortic tissues (F) and densitometric analysis (H, n = 4). (G, I) Representative Western blot bands of EBI3 and IL‐12A in HUVECs (G) and densitometric analysis (I, n = 4). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 Subunit EBI3 is Downregulated in both Obese Mouse Aortas and PA‐Treated HUVECs, and Restored by Exogenous IL‐35. (A, B) Immunofluorescence of EBI3 (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (C, D) Immunofluorescence of IL‐12A (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (E) Serum IL‐35 concentrations, n = 8. (F, H) Representative Western blot bands of EBI3 and IL‐12A in aortic tissues (F) and densitometric analysis (H, n = 4). (G, I) Representative Western blot bands of EBI3 and IL‐12A in HUVECs (G) and densitometric analysis (I, n = 4). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: HUVECs were assigned to the following groups: control, PA (100 μM), PA + IL‐35 (50 ng/mL), PA + IL‐35 (100 ng/mL), PA + IL‐35 + sorafenib (5 μmol/L, Raf inhibitor, MCE, USA), PA + IL‐35 + SCH772984 (0.5 μmol/L, ERK inhibitor, MCE, USA), PA + IL‐35 + si‐NC (50 nM), and PA + IL‐35 + si‐STAT3 (50 nM), with a total treatment duration of 24 h. PA, IL‐35 and inhibitors were added simultaneously for the control, PA, PA + IL‐35, PA + IL‐35+sorafenib and PA + IL‐35 + SCH772984 groups.

    Techniques: Immunofluorescence, Fluorescence, Western Blot, Analysis

    IL‐35 Restores eNOS Ser1177 phosphorylation and nitric oxide production to enhance endothelium‐dependent relaxation in obesity. (A) Concentration‐response curves of endothelium‐dependent relaxation to ACh and comparison of maximal relaxation (Emax) in mouse aortic rings, n = 4. (B) Concentration‐response curves of endothelium‐independent relaxation to SNP and comparison of Emax, n = 4. (C, D) Western blot bands of p‐eNOS (Ser1177) and total eNOS in mouse aortic tissues (C) and HUVECs (D). (E, F) Densitometric analysis of total eNOS and p‐eNOS (Ser1177)/total eNOS in aortic tissues (E, n = 4) and HUVECs (F, n = 4). (G) NO fluorescence intensity in HUVECs, scale bar = 50 μM. (H) Semi‐quantification of NO fluorescence intensity in HUVECs, n = 3. Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 Restores eNOS Ser1177 phosphorylation and nitric oxide production to enhance endothelium‐dependent relaxation in obesity. (A) Concentration‐response curves of endothelium‐dependent relaxation to ACh and comparison of maximal relaxation (Emax) in mouse aortic rings, n = 4. (B) Concentration‐response curves of endothelium‐independent relaxation to SNP and comparison of Emax, n = 4. (C, D) Western blot bands of p‐eNOS (Ser1177) and total eNOS in mouse aortic tissues (C) and HUVECs (D). (E, F) Densitometric analysis of total eNOS and p‐eNOS (Ser1177)/total eNOS in aortic tissues (E, n = 4) and HUVECs (F, n = 4). (G) NO fluorescence intensity in HUVECs, scale bar = 50 μM. (H) Semi‐quantification of NO fluorescence intensity in HUVECs, n = 3. Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: HUVECs were assigned to the following groups: control, PA (100 μM), PA + IL‐35 (50 ng/mL), PA + IL‐35 (100 ng/mL), PA + IL‐35 + sorafenib (5 μmol/L, Raf inhibitor, MCE, USA), PA + IL‐35 + SCH772984 (0.5 μmol/L, ERK inhibitor, MCE, USA), PA + IL‐35 + si‐NC (50 nM), and PA + IL‐35 + si‐STAT3 (50 nM), with a total treatment duration of 24 h. PA, IL‐35 and inhibitors were added simultaneously for the control, PA, PA + IL‐35, PA + IL‐35+sorafenib and PA + IL‐35 + SCH772984 groups.

    Techniques: Phospho-proteomics, Concentration Assay, Comparison, Western Blot, Analysis, Fluorescence

    IL‐35 induces the MAPK/ERK signaling pathway, thereby enhancing eNOS Ser1177 phosphorylation and NO production in endothelial cells. (A) Volcano plot of eNOS‐interacting proteins in HUVECs (PA vs. PA + IL‐35) by IP‐mass spectrometry. Red dots: upregulated in PA + IL‐35 group (fold change > 1.5, p < 0.05); MAPK3 (ERK1) is highlighted. (B) STRING‐based PPI network of top 20 binding proteins. ERK1 is the only direct interactor with eNOS. (C) Western blot bands of p‐cRAF, p‐MEK, p‐ERK, p‐eNOS (Ser1177), and total proteins in HUVECs. (D–G) Densitometric analysis of p‐cRAF, p‐MEK, p‐ERK, and p‐eNOS (Ser1177) normalized to total proteins, n = 3. (H, I) NO fluorescence intensity in HUVECs (H, scale bar = 50 μM) with semi‐quantification (I, n = 3). Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 induces the MAPK/ERK signaling pathway, thereby enhancing eNOS Ser1177 phosphorylation and NO production in endothelial cells. (A) Volcano plot of eNOS‐interacting proteins in HUVECs (PA vs. PA + IL‐35) by IP‐mass spectrometry. Red dots: upregulated in PA + IL‐35 group (fold change > 1.5, p < 0.05); MAPK3 (ERK1) is highlighted. (B) STRING‐based PPI network of top 20 binding proteins. ERK1 is the only direct interactor with eNOS. (C) Western blot bands of p‐cRAF, p‐MEK, p‐ERK, p‐eNOS (Ser1177), and total proteins in HUVECs. (D–G) Densitometric analysis of p‐cRAF, p‐MEK, p‐ERK, and p‐eNOS (Ser1177) normalized to total proteins, n = 3. (H, I) NO fluorescence intensity in HUVECs (H, scale bar = 50 μM) with semi‐quantification (I, n = 3). Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: HUVECs were assigned to the following groups: control, PA (100 μM), PA + IL‐35 (50 ng/mL), PA + IL‐35 (100 ng/mL), PA + IL‐35 + sorafenib (5 μmol/L, Raf inhibitor, MCE, USA), PA + IL‐35 + SCH772984 (0.5 μmol/L, ERK inhibitor, MCE, USA), PA + IL‐35 + si‐NC (50 nM), and PA + IL‐35 + si‐STAT3 (50 nM), with a total treatment duration of 24 h. PA, IL‐35 and inhibitors were added simultaneously for the control, PA, PA + IL‐35, PA + IL‐35+sorafenib and PA + IL‐35 + SCH772984 groups.

    Techniques: Phospho-proteomics, Mass Spectrometry, Binding Assay, Western Blot, Analysis, Fluorescence

    IL‐35 activates the STAT3‐MAPK/ERK‐eNOS signaling axis to promote eNOS phosphorylation and NO production in endothelial cells. (A) Western blot bands of p‐STAT3, p‐eNOS (Ser1177), p‐cRAF, p‐MEK, p‐ERK, and corresponding total proteins in HUVECs. (B–I) Densitometric analysis of p‐STAT3, STAT3, p‐eNOS (Ser1177), eNOS, p‐cRAF, p‐MEK, and p‐ERK normalized to total proteins or β‐tubulin, n = 3. (J–K) NO fluorescence intensity in HUVECs (J, scale bar = 50 μM) with semi‐quantification (K, n = 3). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 activates the STAT3‐MAPK/ERK‐eNOS signaling axis to promote eNOS phosphorylation and NO production in endothelial cells. (A) Western blot bands of p‐STAT3, p‐eNOS (Ser1177), p‐cRAF, p‐MEK, p‐ERK, and corresponding total proteins in HUVECs. (B–I) Densitometric analysis of p‐STAT3, STAT3, p‐eNOS (Ser1177), eNOS, p‐cRAF, p‐MEK, and p‐ERK normalized to total proteins or β‐tubulin, n = 3. (J–K) NO fluorescence intensity in HUVECs (J, scale bar = 50 μM) with semi‐quantification (K, n = 3). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: HUVECs were assigned to the following groups: control, PA (100 μM), PA + IL‐35 (50 ng/mL), PA + IL‐35 (100 ng/mL), PA + IL‐35 + sorafenib (5 μmol/L, Raf inhibitor, MCE, USA), PA + IL‐35 + SCH772984 (0.5 μmol/L, ERK inhibitor, MCE, USA), PA + IL‐35 + si‐NC (50 nM), and PA + IL‐35 + si‐STAT3 (50 nM), with a total treatment duration of 24 h. PA, IL‐35 and inhibitors were added simultaneously for the control, PA, PA + IL‐35, PA + IL‐35+sorafenib and PA + IL‐35 + SCH772984 groups.

    Techniques: Phospho-proteomics, Western Blot, Analysis, Fluorescence

    Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Concentration-dependent change in the humoral inflammatory response following incubation with Escherichia coli ( E. coli ) in the ex vivo whole blood model. a Absolute plasma concentrations of IL-6, IL-8, and MMP9 determined by enzyme-linked immunosorbent assay. b Normalized values and EC 50 curve fit by BuC=0% and 50 000 CFU/ml E. coli= 100%, respectively, for IL-6, IL-8, and MMP9 as indicated by EC 50 (%) on the respective Y-axis. BuC indicates buffer control after 60 min incubation; numbers on the X-axis indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation; LPS indicates lipopolysaccharide (LPS) 100 ng/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing all shown concentrations of E. coli bacteria and 100 ng/ml LPS with BuC. P -values are indicated above the respective data points. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Concentration Assay, Incubation, Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Bacteria

    Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Diagnostic performance for the detection of bacteremia, analyzing the neutrophil phenotype by determining the median fluorescence intensity (MFI) and the cellular response capacity (CRC) in comparison with traditional markers of humoral inflammation (IL-6, IL-8, MMP9). a Comparison of receiver operating characteristic (ROC) at 10,000 CFU/ml Escherichia coli ( E. coli ) with the respective 95% confidence interval (CI) and P -value, and half-maximal effective concentration (EC 50 ) as a function of the E. coli concentration. b Detailed comparison of the EC 50 as a function of the E. coli concentration. c Exemplary comparison of EC 50 curve fit after normalization as indicated by EC 50 (%) on the respective Y-axis to BuC=100% and 50 000 CFU/ml E. coli =0% for the humoral marker IL-6 (the IL-6 values were multiplied by −1 before EC 50 calculation to facilitate comparability with the CRC) and the change in neutrophil phenotype represented by CD11b CRC. BuC indicates buffer control after 60 min incubation; numbers on the X-axis of c indicate E. coli bacteria in concentrations of 2000 to 50 000 CFU/ml after 60 min incubation. Values are shown as median and interquartile range. n =8. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, evaluating the EC 50 of IL-8, MMP9, the MFI, and CRC of CD10, CD11b, and CD62L in comparison to the EC 50 of IL-6. P -values are indicated above the respective data points. ⁎ P <0.05. CFU. Colony-forming units; IL. Interleukin; MMP9. Matrix metallopeptidase 9.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Diagnostic Assay, Fluorescence, Comparison, Concentration Assay, Marker, Control, Incubation, Bacteria

    Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Clinical specifications and parameters over all time points of the sepsis cohort. a Suspected infection cause of sepsis. b Distribution of the individual score points of the Sequential Organ Failure Assessment (SOFA) score. c Total SOFA score. d-h Traditional and humoral markers of inflammation: leukocytes and neutrophil-lymphocyte ratio ( d ), C-reactive protein (CRP) and procalcitonin (PCT) ( e ), interleukin-6 (IL-6) and interleukin-8 (IL-8) ( f ), serum amyloid A (SAA) and calprotectin ( g ), matrix metallopeptidase 9 (MMP9) and myeloperoxidase (MPO) ( h ). Values are shown as median and interquartile range. n =14. CNS. Central nervous system; HV. Healthy volunteers.

    Article Snippet: For the samples of the ex vivo whole blood model, the plasma concentrations of matrix metallopeptidase 9 (MMP9, #DY911, R&D Systems, Minneapolis, USA), IL-6 (#555220, BD Biosciences, San Jose, USA), and IL-8 (#DY208, R&D Systems) were measured in citrate-anticoagulated plasma using enzyme-linked immunosorbent assay according to the respective manufacturer’s instructions.

    Techniques: Infection

    Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin (IL)-4/IL-13 or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.

    Journal: Regenerative Therapy

    Article Title: Prednisolone attenuates the immunomodulatory capacity and reshapes the functional phenotype of canine adipose-derived mesenchymal stromal cells

    doi: 10.1016/j.reth.2026.101184

    Figure Lengend Snippet: Effects of prednisolone on macrophage polarization and regulatory T (Treg) cell induction mediated by cADSCs. (a) Representative flow cytometry plots showing cluster designation (CD) 80 and CD206 expression in CD14 + DH82 cells. Cells cultured alone under control conditions or stimulated with interleukin (IL)-4/IL-13 or lipopolysaccharide (LPS)/interferon (IFN)-γ are shown, together with DH82 cells co-cultured with cADSCs pretreated with prednisolone at the indicated concentrations (0–100 μM) under LPS/IFN-γ stimulation. (b) Quantitative analysis of macrophage subsets, defined as CD14 + CD80 + CD206 - , CD14 + CD80 − CD206 + , CD14 + CD80 + CD206 + , and CD14 + CD80 − CD206 - cells. IL-4/IL-13 stimulation was included as an M2-polarizing control, whereas LPS/IFN-γ stimulation induced M1-like polarization. (c) Representative flow cytometry plots showing CD25 and Foxp3 expression in CD4 + T cells after co-culture of PBMCs with cADSCs pretreated with prednisolone. Red boxes indicate CD25 + Foxp3 + Treg cells. (d) Quantification of CD25 + Foxp3 + Treg cells within the CD4 + T-cell population. Data are presented as mean ± SD from three independent experiments using cADSCs derived from three independent donors. * p < 0.05 vs. control or PBMCs alone; † p < 0.05 vs. LPS/IFN-γ.

    Article Snippet: To induce macrophage polarization, either a combination of 50 ng/mL lipopolysaccharide (LPS; Sigma-Aldrich) and 20 ng/mL recombinant canine interferon (IFN)-γ (R&D Systems), or a combination of 40 ng/mL recombinant human interleukin (IL)-4 (Thermo Fisher Scientific) and 40 ng/mL recombinant human IL-13 (Thermo Fisher Scientific), was added to the cultures, followed by incubation at 37 °C in 5% CO 2 for 72 h. At the end of the co-culture period, cells were collected, washed with FACS buffer, and incubated on ice with canine Fc receptor binding inhibitor.

    Techniques: Flow Cytometry, Expressing, Cell Culture, Control, Analysis, Co-culture Assay, Derivative Assay